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An easy, rapid and sensitive novel spectrophotometric method for estimation of trace amounts of Promethazine-HCl in aqueous solution is described. The method is based on using the oxidative coupling reaction of Promethazine-HCl with m-amino benzoic acid reagent at pH1.2 in the presence of N-bromosuccinimide to produce an intense green color, water soluble, product which exhibit maximum absorbance at 592 nm. Beer's law is obeyed over concentration range of (2-26 μg/ml), with a Ԑ 9.754 l/mol.cm, Coefficient determination (R 2 =0.9999 . a   i . 9 , the average recovery is 100.396 %, RSD does not exceed 0.3996 %, LOD of 0.1949 μg/m1 and LOQ of 0.5905 μg/m1. The proposed method was applied successfully for determination of PMZH in its pharmaceutical formulations.

maǧallaẗ ǧāmiʻaẗ kirkūk. al-dirāsāt al-ʻilmiyyaẗ/mağallaẗ ğāmiʿaẗ kirkūk. al-dirāsāt al-ʿilmiyyaẗ

Spectrophotometric Method for Estimation of Promethazine- HCl in Pharmaceutical Formulation Based on Oxidative Coupling with the Reagent m-Amino Benzoic Acid in Presence of N-Bromosuccinimide.