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The effect of nucleotides in the newborn is a determinant in this first stage of life, and their correct level in breastmilk is vital. We have designed a new method for the assay of nucleotides in milk by capillary electrophoresis (CE) after acid hydrolysis. Breastmilk samples were collected from healthy mothers (ages, 25–35 years) of one month lactation, and stored at -20 °C. The duplicated samples were dissociated by acidic hydrolysis (HClO4) and the CE assay was performed in an uncoated fused-silica capillary using an alkaline (borate) electrophoretic separation system. The method gave good recoveries of 5 ́-mononucleotides. Under the conditions used, the actual CE analysis time was less than 20 minutes. The physiologically and nutritionally important nucleotides were detected at concentrations of 387 μg/100ml for UMP-5P, 385.3 μg/100ml for AMP-5P, 67 μg/100ml for CMP-5P, 172 μg/100ml for TMP-5P and 315 μg/100ml for GMP-5P. Nucleotides are a significant nutrient in infant growth, and capillary electrophoresis is a sensitive and efficient tool for the assay of nucleotides with a purine or pyrimidine base in breastmilk.

A new analytical technique in capillary electrophoresis: studying the levels of nucleotides in human breastmilk