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Objectives: the name of Jan Evangelista Purkyně (Purkinje in German), born in Bohemia in 1787 and died in Prague in 1869, is mainly associated with discoveries in histology and specialist fields of Medicine like embriology, histological techniques, ophthalmology, cardiology and neurophysiology. This short article presents a brief account of his life, commemorates his achievements in biology and medicine but also in in the politics and literature of his Country (he was elected to the Diet of Bohemia but also he composed poems and important translations from German, French and Italian languages into Czech) and examines in depth his contribution to Dentistry. Materials and Methods: Purkyně’s major contributions to Dentistry, which focused on embryology and dental histology, endodontics and periodontology, are traced to two dissertations in Latin which were discussed by his pupils (Meyer Fraenkel and Isaac Raschkow), at Breslau University in 1835: we present a brief summary of each, with the major innovative findings highlighted. Results: the two dissertations contain remarkable, though often overlooked, contributions to Dentistry. Among these we can indicate the individuation of: the dental cement (substantia ostoidea), the acquired dental pellicle, the nature of optical illusion of Hunter-Schreger lines, the “enamel pulp” from which the enamel would evolve, the sub-odontoblastic nervous plexus which is the cause of tooth sensitivity, the predentine, the organic nature of the process of enamel formation, the dentine and enamel  formation in opposing directions, the presence of alveolus membrane (id est: the periodontium). Conclusions: after reviewing the main innovations these two dissertations made to Dentistry, Purkyně’s personal share in both is very clear. Both the two his pupils acknowledged their debt to Purkyně and also famous contemporary Purkinje scientists such as Alexander Nasmyth, Sir Richard Owen, Sir James Paget had no doubt he is had generated the ideas expressed in the two little treatises.

The forgotten contribution of J. E. Purkyně to dentistry