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H epatitis c is one of the most dangerous and widespread viral diseases. currently, the World Health Organization estimates that about 170 million people are infected with the hepatitis c virus (HcV), the causa-tive agent of infection, in almost every country in the world. the rnA-dependent rnA-polymerase (r-rnAP, virus nonstructural protein) is a key fragment that carries out HcV genome replication. r-rnAP is about 65 kDA in molecular weight and localized on the endoplasmic reticulum membrane of infected hepatic cells by the c-tail α-spiral transmembrane domain (21 a.r.). One characteristic feature of r-rnAP is its ability to catalyze rnA synthesis by both primer-dependent and primer-independent (de novo) mechanisms [1]. In the first case, in the in vitro experiments, the primer-matrix poly(rA)-oligo(ru) duplex is used as the rnA matrix; in the second case, the HcV genome fragments are used. It is suggested that oligomer from several identical r-rnAP molecules takes part in the replication. Moreover, oligomer was discovered to be composed of H502 and e18 amino-acid residues located in the interaction area of protein globules [2]. earlier, we obtained the E.coli strain (the HcV r-rnAP producer) which allows a highly purified recombinant protein with a cell culture yield of up to 6 mg/l to be created and we developed a procedure for enzyme purification to the homogeneous condition (the data of electrophoresis in the polyacrylamide gel) [3]. the purification procedure included methods similar to those described in the corresponding literature [4]; the kinetic parameters of the primer-dependent reaction determined for the polymerase sample were consistent with the literature data as well [5]. However, anomalously high incorporation of the radioactive label was noted for the primer-independent replication of the heterogeneous rnA (for instance, (-)IreS matrix, which represents a 3'-nontranslated region of the HcV virus genome chain). the enzyme was also incapable of single-step oligonucleotide elongation in the primer-dependent system and, thus, could not be used to the full extent in investigations of specific in-hibitors. those facts testified to the possible impurities of the rnA cell in the protein sample, which formed a stable complex with the enzyme molecule and could act as an " endogenic " matrix. Hence, the need to modify the enzyme purification method and to more thoroughly determine the parameters of its interaction with the different types of matrices obtained. HcV r-rnAPs were expressed in the Escherichia coli cells using the рЕТ21-2с-5B∆55 plasmid, as was described earlier [3]. Initially, …

The Interaction between the RNA-Dependent RNA-Polymerase of the Hepatitis Virus and RNA Matrices