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In agricultural production, a single insect-resistant and disease-resistant variety can no longer meet the demand. In this study, the expression vector pCAMBIA-3301- PR1  containing the disease-resistant gene  PR1  was constructed by means of genetic engineering, and the  PR1  gene was genetically transformed to contain the  PR1  gene through the pollen tube method. In  CryAb-8Like  transgenic high-generation T 7  receptor soybean, a new material that is resistant to insects and diseases is obtained. For T 2  transformed plants, routine PCR detection, Southern Blot hybridization, fluorescence quantitative PCR detection, indoor and outdoor pest resistance identification and indoor disease resistance identification were performed. The results showed that there were 9 positive plants in the routine PCR test of T 2  generation. In Southern Blot hybridization, both  PR1  and  CryAb-8Like  genes are integrated in soybeans in the form of single copies. Fluorescence quantitative PCR showed that the expression levels of  PR1  and  CryAb-8Like  genes are different in different tissues. The average expression levels of  PR1  gene in plant roots, stems, and leaves are 2.88, 1.54, and 5.26, respectively.  CryAb-8Like  genes are found in roots, stems, and leaves. The average expression levels were 1.36, 1.39, and 4.25, respectively. The insectivorous rate of the  CryAb-8Like  gene in outdoor plants with positive insect resistance identification was 3.78%. The disc partition method was used indoors for pest resistance identification, and the bud length of transformed plants increased significantly. The average mortality rate of untransformed plants in indoor disease resistance identification was as high as 56.66%, and the average mortality rate of plants transformed with  PR1  gene was 10.00%, and disease resistance was significantly improved. Therefore, a new material with resistance to diseases and insects is obtained.

Bioinformatics Analysis of Disease Resistance Gene PR1 and Its Genetic Transformation in Soybeans and Cultivation of Multi-resistant Materials