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The definition of relatively stable expressed internal reference genes is essential in both traditional blotting quantification and as a modern data quantitative strategy. Appropriate internal reference genes can accurately standardize the expression abundance of target genes to avoid serious experimental errors. In this study, the expression profiles of ten candidate genes,  ACT1 ,  ACT2 ,  GAPDH ,  eIF1 ,  eIF2 ,  α-TUB ,  β-TUB ,  TBP ,  RNA Pol II  and  RP II , were calculated for a suitable reference gene selection in  Paeonia ostii  T. Hong et J. X. Zhang leaves under various drought stress conditions. Data were processed by the four regularly used evaluation software. A comprehensive analysis revealed that  RNA Pol II  was the most stable gene and  eIF2  was the least stable one. In addition, the geNorm program provided the optimal choice of two reference gene combination,  RNA Pol II  and  β-TUB , for qRT-PCR normalization in  P. ostii  subjected to different drought stress levels. Our research provided convenience for gene expression analysis in  P. ostii  under drought stress and promoted research of effective methods to alleviate  P. ostii  drought stress in the future.

phyton-international journal of experimental botany

Selection of Stable Reference Genes for Quantitative Real-Time PCR on  Paeonia ostii  T. Hong et J. X. Zhang Leaves Exposed to Different Drought Stress Conditions