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The present study aimed to develop a protocol for somatic embryogenesis and encapsulation of coffee embryos ( Coffea arabica  L.), for the conservation of genotypes with characteristics of commercial interest. Somatic embryos were induced from leaf explants in Murashige and Skoog medium (MS) supplemented with 1 mg · L −1  of 2,4-dichlorophenoxiacetic acid (2,4-D) combined with 2 mg · L −1  of benzyladenine (BA). Somatic embryos (SE) at the globular stage were encapsulated in a sodium alginate matrix; two treatments were tested: MS + 5 mg · L −1  BA + 1 mg · L −1  NAA + 3% (w/v) alginate, and MS + 7 mg · L −1  BA + 5.7 mg · L −1  indoleacetic acid (IAA) + 3% (w/v) alginate. Alginate was complexed with 100 mM calcium chloride (CaCl 2 ). Viability of the encapsulated SE was determined by staining with 0.01% fluorescein diacetate (FDA) after 0, 15, 30, and 45 days of storage at 4°C. Embryo viability was 100% in both treatments.

Encapsulation of Immature Somatic Embryos of Coffea arabica L. for in Vitro Preservation