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Antagonistic bacteria used as biological control agentmay loss effectiveness at the field due to environmental factors suchas UV radiation, dryness and high temperature. An inexpensive alternativeto protect antagonistic bacteria against such factors is theuse of microencapsulating agents. In this work, the effect of microencapsulationof Bacillus subtilis with commercial gums on theirantagonistic capacity against Meloidogyne incognita was evaluated.The efficiency of the microencapsulation was verified by the differencebetween the initial and final concentrations of protein release.The effectiveness as antagonist was evaluated against M. incognita intomato under greenhouse conditions. The microcapsules based oncarboxymethylcellulose (MBC) and xanthan (MBX) were morphologicallydifferent. The MBX showed a higher bacterial release efficiency(90.2%) compared to that of MBC (76.6%). Plants inoculatedwith MBX showed a significant decrease in galls and M. incognitaeggs in comparison to control plants, but this decrease did not occuron those inoculated with non-microencapsulated B. subtilis. The applicationof MBX to tomato plants at transplanting time providedgood protection against M. incognita under greenhouse conditions.

Polymer-based encapsulation of Bacillus subtilis and its effect on Meloidogyne incognita in tomato