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Background    Puerarin (Pue) has been reported to be a natural active ingredient with multiple antifibrotic properties. This work aimed at exploring the function of Pue in oral submucous fibrosis (OSF) treatment.Methods    Human oral mucosa fibroblasts (hOMF) were induced with transforming growth factor beta1 (TGF-β1) and intervened with Pue. Expressions of fibrosis-related markers were analyzed by Western blot and IF staining. Cell viability was characterized by the CCK-8 assay. Expressions of miR-30 family members were quantified by qRT-PCR. The correlation between fibroblast activation protein (FAP) and miR-30 family expression was evaluated by the Pearson correlation coefficient. Bioinformatics prediction and dual-luciferase reporter assay were employed to verify the regulation between FAP and miR-30b-5p. The specific mechanism of Pue on OSF was explored through the promotion or inhibition of miR-30b-5p.Results    After induction by TGF-β1, hOMF showed upregulated Collagen I, Collagen III, and FAP expressions, while miR-30 family expression was downregulated with miR-30b-5p being the most significant. Pue intervention inhibited the excessive proliferation of TGF-β1-induced hOMF, downregulated FAP, collagen type 3 (COL3A1), collagen type 1 (COL1A1), matrix metalloproteinase 1 (MMP1), and matrix metalloproteinase 3 (MMP3) expressions, and restored miR-30 family expression. Bioinformatics prediction and dual-luciferase reporter assay revealed that miR-30b-5p selectively inhibited FAP expression. Mechanistically, miR-30b-5p mimic suppressed the excessive proliferation of TGF-β1-induced hOMF and declined fibrosis levels. Pue intervention significantly reversed the promotion of TGF-β1-induced OSF by miR-30b-5p inhibition.Conclusion    Pue mediated miR-30b-5p targeting FAP against OSF, which provided a theoretical basis for the pathogenesis research and Pue application in OSF.

Puerarin mediated miR-30b-5p targeting fibroblast activation protein against oral submucous fibrosis