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Background: Organic agricultural pesticides are so stable in ecosystems and biosphere. These compounds affect soil conditions and agricultural products. The aim of this study was isolation of diazinon-degrading bacteria from Kerman pistachio orchards, Southeastern Iran.  Methods: Diazinon-degrading bacteria were enriched in Bushnell-Hass medium. Identification and sequencing of prevalent degrading strains was performed using PCR based on amplifying of 16s rDNA.  Results: The soil of pistachio orchard has some degrading bacteria that are suitable for elimination of diazinon from soil and environment. Three diazinon-degrading bacteria strains belong to: Pseudomonas putida strain D3, P. fluorescens strain D1 and Achromobacter piechaudii strain D8. The best degrading strain (D1), up to 100 ppm, illustrated a good growth, whereas more than this concentration, the growth was reduced. The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.  Conclusion: The results of Gas-Chromatography (GC) confirmed the decomposition of organic pesticide by degrading-bacteria.

Isolation and Characterization of Diazinon Degrading Bacteria from Contaminated Agriculture Soils