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Background and Aim: The bleached enamel surface requires application of antioxidant as there will be continuous release of free oxygen radicals. It would be beneficial if remineralizing agent which is recommended after bleaching also provides an antioxidant therapy. The natural antioxidants are colored. Hence, the aim of the present study was to evaluate the effect of natural antioxidants on the shade of the bleached enamel. Materials and Methods: Thirty single rooted maxillary anterior teeth were selected for the study. For all the samples middle third portion of the labial surface of the teeth was standardized using computerized cellophane stickers. Bleaching was carried out with 22% Carbamide peroxide gel (Philips Zoom Nite White). Based on the type of antioxidant solution application, samples were divided into three groups of ten samples each. Group I control (n=10) Group-II (n=10) 10% wheat grass solution (Sresta natural Bio products, India) Group-III (n=10) 5% grape seed extract (Zenith nutrition, Medizen Labs Pvt.Ltd, India). L, a, b values were recorded with spectrophotometry (Vita Zahnfabrik H. RauterGmbH & Co. KG,Bad Sackingen, Germany) thrice for each sample at the three respective time periods i.e at baseline, after bleaching and after antioxidant application. Results: ΔE values were subjected to statistical analysis using one way ANOVA with p>0.05 and the results were tabulated. It was observed that there was statistical insignificant difference between the groups with a p value of 0.295(p>0.05). Conclusion: The antioxidants 5% grape seed extract and 10% wheat grass extract did not affect the shade of the bleached enamel. Keywords: tooth bleaching, antioxidant, shade, spectrophotometry

EVALUATION OF EFFECT OF NATURAL ANTIOXIDANTS ON THE SHADE OF THE BLEACHED ENAMEL USING SPECTROPHOTOMETRY