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People in West Kalimantan are widely using kaffir lime leaves (Citrus hystrix) as a spice in cooking. The leaves have a distinctive aroma and have the potential to be processed into essential oils and used as a natural antibacterial. This study aims to analyze the essential oils bioactivity of kaffir lime leaves against bacteria of Propionibacterium acnes and Pseudomonas aeruginosa. The research method is by steam distillation for 5 hours, and testing for antibacterial activity using four levels of oil concentration that is 0.5; 1; 5 and 10%. The yield of oil produced was quite high, at 1.083%. Kaffir lime leaf oil has low bioactivity in inhibiting the growth of bacteria P. acnes and P. aeruginosa with inhibition zones formed at a concentration of 10% are 1.8 mm (P. acnes) and 1.36 mm (P. aeruginosa).

MINYAK ATSIRI DAUN JERUK PURUT (Citrus hystrix) DAN BIOAKTIVITASNYA TERHADAP BAKTERI Propionibacterium acnes DAN Pseudomonas aeruginosa