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The objective of the present study was to extend the knowledge of the biology of the parasitoid Nasonia vitripennis (Walker) (Hymenoptera: Pteromalidae). The pupae were obtained using the flotation method. They were individually placed in gelatin capsules until the emergence of adult dipterous or their parasitoids. Between May 1998 and February 2013, 6.030 fly pupae were collected. In 228 of these pupae, 1105 specimens of N. vitripennis were found. The overall percentage of parasitism was 3.8%. Chrysomya megacephala (Fabricius) (Diptera: Calliphoridae) was the fly that had the highest percentage parasitism (9.6%). In relation to the hosts collected, the species Chrysomya albiceps (Wiedemann) (Diptera: Calliphoridae), C. megacephala and Musca domestica L. (Diptera: Muscidae) medical-veterinary. Keywords: Hymenoptera, Diptera, substrates, traps, baits.

Parasitoid flies collected in the south Goiás and west Minas Gerais, Brazil