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This paper presents a novel diagnostic method using a library of molecular receptors to assess the composition of healthy and tumorous tissue samples. Molecular receptors were formed by self-organization of N-lipidated peptides attached in the regular fashion via arninophenylamino-1,3,5-triazine linkers to the surface of a cellulose plate. The library was cloned into multiple, identical copies. A new clone was used in each test. The binding process was monitored by staining the discs with Brilliant Black and quantitative color measurement was performed in 256-grade grayscale. Substantial differences in the composition of healthy and tumor samples were observed in most cases. The sharp differences between individual chemical fingerprints were found to depend on the cancer type. Statistical analysis identified the two most useful receptors for diagnosing malignant papillary thyroid cancer.

Molecular Fingerprints of Thyroid Cancer Cells by Using Library of Molecular Receptors Formed by N-Lipidated Peptides Immobilized on Cellulose