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BACKGROUND: We use a temporal template method, the motion history image (MHI), to visualize the hypoperfusion (decreased blood flow) during an acute cerebral ischemic event in a mouse brain. The MHI method was implemented on the dynamic fluorescent (DF) data images. AIMS: Our aim was to implement the MHI method on the DF imaging data and visualize the regions where perfusion evolves with time. METHODOLOGY: The MHI method was used to process the DF data images recorded during an acute cerebral ischemic event in a mouse brain. RESULTS: We demonstrate that, the MHI images clearly illustrates the locations where perfusion decreases during occlusion and that is more easily obtained in comparison to a visual inspection of all of the raw DF images constituting the recordings. CONCLUSION: MHI can be a useful tool for the clinical and research studies.

biomedical spectroscopy and imaging

Application of motion history image (MHI) on dynamic fluorescent imaging for monitoring cerebral ischemia induced by occlusion of middle cerebral artery (MCA) in mouse brain