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In 25 taxa of Picea the somatic chromosomes were analyzed by a fluorescent banding technique using chromomycin A3 (CMA) and DAPI. They had 2n=24 chromosome number and similar karyotypes among species. On metaphase chromosomes in each taxon the CMA-bands appeared at the interstitial and centromeric regions. DAPI-bands appeared on chromosomes of some species. The CMA-band pattern of each taxon was described after identification of most homologous chromosomes and compared among taxa. An alignment of species based on CMA-band pattern with some exception were coincident on their geographic distribution, and discussed on interspecific relationships, comparison to molecular phylogeny and DNA sequences corresponding to fluorescent bands.

Chromosome banding in <i>Picea</i> IV. Comparative karyotype analysis of fluorescent band patterns in 25 taxa