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Allium altaicum Pall. collected in Mts. Altai, Altai Republic, Russian Federation was readily cross-hybridized with A. fistulosum L. a Japanese cultivar ‘Matsushiro-Ippon-Negi,’ and studied to find genic relationships between them. The both parents and all of the hybrid progenies had the common karyotype of 2n=16=12m+2sm+2st and the purplish color around the leaf base that was a characteristic of A. altaicum. Amplified PCR products were performed onto an agarose gel (1.5% w/v) electrophoresis in 1xTAE buffer at 100V for 30 min. The gel was visualized by ethidium bromide staining and photographed under UV illuminator. Each of the species and hybrid offsprings studied by RAPD was indeed, species and the exactly cross-hybrids plants.

chromosome botany

Biosystematic comparisons of &lt;i&gt;Allium altaicum&lt;/i&gt; in Altai Republic, Russian Federation and Mongolian People’s Republic and &lt;i&gt;A. fistulosum&lt;/i&gt; cv. ‘Matsushiro-Ippon-Negi’