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Passionfruit is a species susceptible pathogens such as Fusarium solani f . sp . passiflorae, causal agent of Passiflora collar rot, which can cause yield losses between 90 and 100% in commercial crops in Colombia . Due to lack of knowledge of an efficient inoculation method to evaluate germplasm of passionfruit against this fungus, it was designed this study, whose objective was to compare four methods of inoculation of F. solani f . sp . passiflorae MViRi01 in passionfruit seedlings (Passiflora edulis f . flavicarpa) cultivated at 29oC and 12-hours of photoperiod . The treatments were: stem incision (T1), root immersion (T2), stem injection (T3), and a modified test-tube screening methodology (T4) . Disease incidence and incubation period of the fungus was evaluated . The assessed methods showed highly significant differences (P< 0 .001). F. solani f . sp . passiflorae infected roots, stem, and leaves causing stem ascending and descending strangulation . Both T1 (incision) and T4 (screening) treatments showed 100% of disease incidence . The incubation period of the fungus in T4 was 2 .4 days and in T2 (immersion) 19 days . The death of the seedlings occurred 12 days after inoculation in T1 and T3 (injection) treatments . The adventitious root sprouting in the seedlings was induced by injecting (T3) the pathogen . The most suitable methods to inoculate F. solani f . sp . passiflorae on passionfruit seedlings were the modified testtube screening methodology and the stem incision due to its homogeneity and replicability results.

Comparación de métodos de inoculación de Fusarium solani f. sp. passiflorae en plántulas de maracuyá (Passiflora edulis f. flavicarpa)