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The thermosensitivity of two rat glioma cell lines (C6 and 9L) was evaluated with colorimetric microtiter (MTT) assay and colony formation assay (CFA) . The results of 2 assays were compared to assess the reliability of MTT assay for in vitro experiment of hyperthemia. Cultured glioma Cells (C6 and 9L) were exposed to 42, 43 and 44•Ž for 30, 60, 90 and 120 minutes, and the rate of survival was examined by MTT assay and CFA. Their survivals were reduced in a temperature-dependent and heating time-dependent fashion. The results of MTT assay were mostly consistent with those of CFA, although some part of the survival curves did not correlate especially in C6 glioma cells. These results suggest that both C6 and 9L rat glioma cell lines are thermosensitive and MTT assay is a useful method for the evaluation of thermosensitivity in cultured glioma cells.

The thermosensitivity of glioma cells evaluated by MTT assay comparing with CFA.