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The widely described health benefi ts of red beet root (Beta vulgaris L.) have led to increased scientifi c and consumer interest in this vegetable [Chhikara et al., 2019; Clifford et al., 2015; Nemzer et al., 2011]. Red beet is rich in several bioactive compounds which can be useful in the treatment of many diseases, such as hypertension, atherosclerosis, type 2 diabetes, and dementia [Clifford et al., 2015; Nemzer et al., 2011]. Beet root is a rich source of highly bioactive saponins which are associated with haemolytic [Voutquenne et al., 2003]; molluscicidal [Huang et al., 2003]; anti-infl ammatory [da Silva et al., 2002]; antifungal, anti-yeast activity, and antibacterial [de Lucca et al., 2002]; as well as with antiparasitic activity [Traore et al., 2000]. They exhibit also cytotoxic, antitumoric [González et al., 2003], and antiviral effects [Gosse et al., 2002]. Saponins are divided into two groups: steroidal saponins and triterpenoid saponins. Some authors distinguish a third group called steroidal amines or steroidal alkaloids [Bruneton, 1995]. Triterpenoid saponins are a common group of saponins consisting of a triterpenoid aglycone containing 30 carbon atoms and comprising a pentacyclic structure [Sparg et al., 2004]. Triterpene saponins are glycosides

High-Speed Counter-Current Chromatography in Separation and Identification of Saponins from Beta vulgaris L. Cultivar Red Sphere