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Field isolates and laboratory mutants of Botrytis cinerea, which were collected from different regions in Japan, were crossed in various combinations to produce the sexual stage in vitro. Out of 239 isolates of B. cinerea used in this study, 77 isolates produced numerous and well-developed sclerotia on MGA plates at 12•Ž in the dark for about 4 weeks. The sclerotia of the 77 isolates were fertilized by conidia of 90 isolates as spermatizing parents, which formed numerous conidia on PSA plates. Out of 77•~90 crosses among these isolates, apothecia with ascospores were produced from sclerotia in only 5 crosses. In these crosses, dissimilarity of host plants and/or geographical isolation were noted between both

In Vitro Production of the Perfect Stage by Crossing with Japanese Isolates of Botrytis cinerea.