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pv. eriobotryae was always accompanied by some changes in symptoms. The strain with 85-Md plasmid developed symptoms by inducing callus-like tissue within 2 or 3 weeks after inoculation. On the other hand, the cured strains without 85-Md plasmid remained light brown and slightly swollen appearance at the inoculated parts even 50 days after inoculation. Although the margin of the lesion became definite after that time, the typical cankers with callus-like tissue were not induced. In the present study, the multiplication and structural changes were compared in the loquat stems inoculated with a strain that harboured an 85-Md plasmid or a cured strain for 40 days after inoculation, in order to gain information concerning the role of the 85-Md plasmid. A derivative strain NAE34P+ that still harboured an 85-Md plasmid and a derivative strain NAE34Pcured of 85-Md plasmid obtained from P. syringae pv. eriobotryae NAE34 by subculturing five times at 32•Ž were used in this experiment. No difference between parental strain NAE34 and the derivative strain NAE34P+ was detected in pathogenicity and other physiological characteristics. Two derivative strains were prototrophs. To investigate the multiplication of the bacteria in YP medium1), the fresh suspensions (5ml) of the derivative strain NAE34P+ and NAE34Pwere cultured at 25•Ž by shaking. Each 0.1ml of culture suspension was taken out at 12hr intervals and serially diluted with sterile distilled water. One-tenth milliliter of each dilution was spread on YP agar medium1) and incubated at 25•Ž. Colonies were counted after 2 days of incubation. Inocula were prepared by suspending bacteria grown on YP agar medium in distilled water to give a concentration of about 109cells/ml. A drop of the bacterial suspension was placed on the growing young stems of loquat and pricked into the stem with a needle. The inoculated plants were placed in a growth chamber maintained at 25•Ž with a 12hr photoperiod at 30,000lux. At intervals of 1, 10, 20, 30 and 40 days after inoculation, the inoculated parts of the stems were cut. The surface of cut stems were sterilized with a 70% ethanol and 3% sodium hypochlorite solution. After rinsing in sterile distilled water, the cut stems were ground in 5ml sterile distilled water. The suspensions thus prepared were serially diluted and plated on YP agar medium. The plates were incubated at 25•Ž, and the colonies were counted after 3 days. For electron microscopy, samples were taken from the surrounding tissue of the inoculated sites at 10, 20, 30 and 40 days after inoculation. These samples were fixed in 2% glutaraldehyde in a 0.1M

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Ultrastructural Investigation of Loquat Stems Inoculated with a Strain Harbouring an 85-Megadalton Plasmid and a Cured Strain of Pseudomonas syringae pv. eriobotryae.