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Incubation conditions for double antibody sandwich method of ELISA have been evaluated for detection of watermelon mosaic virus (WMV) and cucumber mosaic virus (CMV). When adsorbing specific antibody (globulin) to plate wells, there were no clear differences in ELISA absorbance values for WMV at three incubation temperatures of 6, 27 and 37C. However, when adding test samples to plate wells, combination incubation of 27C for 2hr and 6C for 14hr resulted in maximum values for the two viruses. In adding conjugated globulin to plate wells, higher values were obtained when incubated at 27C than at 37C for 3 or 4hr for WMV and for 1 to 4hr for CMV. Incubation at 6C showed lower values than those obtained at 27 or 37C for 1 to 4hr incubation times. Incubation at 27C was preferable over the conventional incubation temperature of 37C for detection of the two viruses in crude or purified preparations by ELISA.

A Modified Incubation Condition of Enzyme-linked Immunosorbent Assay for Detection of Watermelon Mosaic and Cucumber Mosaic Viruses