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A new bacterial disease was found on leaves of egg plant grown under vinyl-house condition in winter of 1969 in Tokushima prefecture, Japan. The disease usually occurred on leaves, peduncles, and buds, and rarely occurred on stems, petioles, and fruit. The small necrotic spots appeared at first and they fused into large lesions with irregular margin. From this symptom, •gnecrotic leaf spot of egg plant•h was proposed as the disease name. The pathogen attacked egg plant, pot marigold, lettuce, and tomato by artificial spraying inoculation. The bacteriological and serological characteristics of the pathogen were determined. The bacterium was aerobic, Gram negative, and motile by 1 to 7 polar flagella. It produced acid on Hugh-Leifson medium only under aerobic condition. It grew Uschinsky's and Cohn's solution, and did not change Litmus milk. It showed positive reaction in the test of production of green fluorescent pigment, Kovacs' oxidase, and ƒÀ-glucosidase, while negative in arginine dihydrolase, hydrolysis of margarine, and potato rot. It did not produce hydrogen sulfide, levan, and 2-keto gluconate. It produced acid from glucose, fructose, galactose, glycerol, and mannitol, but no acid from sucrose, maltose, lactose, and salicin. It produced alkali from tartrate, malonate, and citrate. It could not grow at 35C. Optimum temperature for growth was 25C and thermal death point was 48C. Antiserum made from living whole cells of the isolate Ep-1, reacted to all of the 5 isolates of the pathogen tested in agar gel diffusion method, but did not react to other pseudomonads. On the basis of bacteriological and serological characteristics and pathogenicity, the present bacterium was identified as Pseudomonas cichorii (Swingle) Stapp 1928. This is the first report of this bacterium to occur on egg plant in the field. Pseudomonas calendulae (Takimoto) Dowson 1943 is thought to be synonym of P. cichorii. (Received September 24, 1977)

Necrotic Leaf Spot of Egg Plant Caused by Pseudomonas cichorii