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The isolates of Pyricularia strains from 16 gramineous and one zingiberaceous species were shake-cultured in a synthetic medium. Acetone precipitates from culture filtrates were subjected to electrophoresis in a horizontal polyacrylamide-gel slab. The zymograms of multimolecular form of peroxidase and nonspecific esterase were compared. The peroxidase zymograms of Pyricularia strains from Oryza sativa, Eleusine coracana, E. indica, E. africana, Setaria viridis var. minor, Phalaris arundinacea, Festuca elatior var. arundinacea, Eragrostis curvula, Lolium multiflorum and Anthoxanthum odoratum were either identical or slightly different in detail. The strains from Leersia oryzoides and Digitaria sanguinalis showed similar zymograms. The strains from Panicum miliaceum, Panicum bisulcatum and Oryza sativa f. fatua indicated similar zymograms which were different from the former two groups. Striking differences were observed among the zymograms of Pyricularia strains from Zingiber mioga, Zizania latifolia and other gramineous plants. The zymogram patterns of nonspecific esterase in the respective strains from Z. mioga, Z. latifolia, P. miliaceum and E. curvula were distinct from others. (Received May 25, 1977)

Comparison of the isozyme patterns of extracellular enzymes in Pyricularia strains from gramineous and zingiberaceous plants.