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A purification method using anti-bacterial sera to remove a major part of the bacterial debris from crude phage suspension followed by differential centrifuging was developed, and was successfully applied to make clear under electron microscope, the shape and size of several phage particles attacking phytopathogenic bacteria.Each of Xanthomonas oryzae phages OP1, OP1h, and OP1h2, the OP1 group ones, is a tadpole shaped particle having a polyhedral head 70mμ in diameter and a tail of 150mμ in length and 15mμ in width. X. oryzae phage OP2 is also tadpole shaped, having a polyhedral head as large as that of OP1, but it differs from OP1 in its long and slender tail of 95mμ in length and 25mμ in width.Xanthomonas citri phage CP1 is also of a tadpole shaped, but the head looks like spherical, 68mμ in diameter, the tail being 160mμ in length and 15mμ width.

An anti-bacterial serum method for the purification of phages which attack phytopathogenic bacteria