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Pyrethroids are ester of dichloro analog compound that called as Permethrin. Permethrin is widely used as pesticide and insecticide, and off-late as an ectoparasiticide. It is majorly used for effective and short term treatment against lice that is usually found in human and animal head and body hair. Further, Permethrin can also be used against infections namely scabies that formed from insects namely Mite in humans and also in human immunodeficiency virus-infected patients. Isomers of Permethrin differ in their toxicity while (1R, 3S)-trans as well as (1R, 3R)cis isomers are typically effective for the insects killing properties. Permethrin is usually available in cream or lotion form of formulation in the market hence; it is applied on the skin as a cream or lotion. Further estimation of Permethrin in an environment samples like Waste/Potable water and pharmaceuticals is important as a control strategy during environment (water pollution) monitoring, formulation batch release and stability study. This research study emphasizes on the development of an Ultra Performance Liquid Chromatography (UPLC) based rapid detection and quantitation with an accurate and precise analytical method for Permethrin and its traces by an Ultra Performance Liquid Chromatography. The external standard method is used for assay determination in Waste/Potable water, and Cream formulation samples. To prove the suitability of the developed analytical method for its intended purpose, its validation study was performed following an International council for Harmonization guidelines.

FAST AND ADVANCED ANALYTICAL METHOD FOR DETERMINATION OF PERMETHRIN (CIS AND TRANS) CONTENT AND IN TRACES BY ULTRA PERFORMANCE LIQUID CHROMATOGRAPHY