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In the current work, iron nanoparticles (Fe-NPs) were prepared from extracts of Ruellia tuberosa L. extracted with ethanol and water, and their antibacterial activities toward pathogenic bacteria (S. aureus and E. coli) were investigated. The morphologies, structures, sizes and distribution of atom on the surface of Fe-NPs were checked by SEM-EDS, UV-Vis and FTIR spectroscopy. The pH conditions (3, 5, 7, and 9) were chosen as experimental factors for the preparation of Fe-NPs. Formation of results in mostly spherical shapes with particle size found within the range 15-50 nm, and the smallest particle size was obtained from pH 9. UV-Vis spectra confirmed that the formation of Fe-NPs emerged in the UV region around 275 nm. FTIR spectra showed the presence of polyphenolic compounds from extracts of R. tuberosa L, that decreasing aggregations of Fe-NPs and act as bioreductors. As Fe(II) reduced to Fe(0), oxidation of O, OH, and CHO, COOH occurred. The optimal condition for antibacterial activities was at pH 3, resulted in the 16.70 and 13.40 mm of inhibition zones, for S. aureus and E. coli, respectively.

PREPARATION OF ANTIBACTERIAL IRON-BASED NANOPARTICLES USING Ruellia tuberosa L. ROOT EXTRACTS AS BIOREDUCTOR