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A novel reversed-phase column was prepared by bio-degradable versatile biopolymer chitosan with 2-Hydroxy napthaldehyde condensed Schiff base (CSb). The prepared stationary phase CSb was characterized by FT-IR and its surface morphology was determined by Scanning electron microscope (SEM). From the SEM outcomes, fluffy and highly porous nature of the CSb was identified. Due to the porous and the insoluble in mobile phase, HPLC column was fabricated using CSb for analytical applications. The stability of the column was tested and found to be stable in acidic and basic medium. Using CSb column, anti-hypertension drug Irbesartan and its potential impurities were confirmed as per ICH guidelines and the results were linear, precise, rugged and accurate. The results of CSb column and commercially available HPLC column outcomes are exhibited similar results but CSb is a long life lowcost stationary phase.

NOVEL HPLC STATIONARY REVERSE PHASE COMPOUND DERIVED TO VALIDATE IRBESARTAN AND ITS IMPURITIES