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It was reported that all fractions of Cinnamomum sintoc methanol extract had antioxidant properties which could delay, slowed down and prevented the occurrence of oxidation reactions caused by free radicals that could trigger degenerative diseases such as cancer, diabetes, and liver. This study aims to isolate and determine chemical compounds from n-hexane fraction of Sintok (C.sintoc Bl.) stem bark. The material was macerated using ethanol 70% followed by fractionation using n-hexane and water solvent. Phytochemical screening was performed to the hexane fraction. Vacuum liquid chromatography (VLC) was used to separate and isolate compounds using and preparative thin layer chromatography. The obtained isolates called FH3-2 and FH3-3 and determined using UV and IR spectrophotometers and Gas Chromatography-Mass Spectrometry. The ultraviolet spectrum of FH3-2 isolate showed the maximum wavelengths of 208, 229, and 275 nm. The infrared spectrum showed the presence of O-H, C-H aromatic groups, C = C, and C-O. The mass spectrum gave m / z: 164 [M+], 149, 131, 121, 103, 91, 77, 55, 51 having 97% similarity with eugenol. The ultraviolet spectrum of FH3-3 isolate showed a maximum wavelength of 206 nm. The infrared spectrum showed the presence of O-H, C = C, and C-O groups. The mass spectrum gave m/z: 222 [M+], 208, 189, 161, 147, 135, 121, 105, 93, 81, 67, 43 having 85% equality with juniper camphor. It could be concluded there were two dominant compounds of the fraction of n-hexane of the Sintoc stem bark i.e eugenol and juniper camphor.

ISOLATION AND DETERMINATION OF CHEMICAL COMPOUNDS FROM N-HEXANE FRACTION OF THE SINTOK STEM BARK (Cinnamomum sintoc Bl.)