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Dragon fruit is one of the fruits cultivated in the tropics. The fruit flesh of the dragon fruit has been widely consumed, and the fruit peel of the dragon fruit has also been extensively utilized. But the leaves of the dragon fruit have not been utilized and tend to be waste in agriculture. This study aims to utilize waste dragon fruit leaves with the test of antioxidant activity and the determination of total phenolic of red dragon fruit leaves extract and white dragon fruit leaves extract by spectrophotometric method. Methods performed for antioxidant activity test by 1,1diphenyl-2-picrylhydrazyl (DPPH) with ascorbic acid as the comparator and total phenolic determination by FolinCiocalteu (FC) with gallic acid as the comparator. Measurements were done with a spectrophotometer. Antioxidant activity test results of red dragon fruit leaves extract and white dragon fruit leaves extract obtained scavenging concentration 50% (SC50) 135.00 μg/mL and 142.47 μg/mL. Total phenolic determination results of red dragon fruit leaves extract and white dragon fruit leaves extract obtained value 756.75 mg/g and 707.07 mg/g. Both red dragon fruit leaves extract and white dragon fruit leaves extract to have moderate antioxidant activity.

SPECTROPHOTOMETRIC METHOD FOR ANTIOXIDANT ACTIVITY TEST AND TOTAL PHENOLIC DETERMINATION OF RED DRAGON FRUIT LEAVES AND WHITE DRAGON FRUIT LEAVES