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Buckeye (Aesculus hippocastanum L.) is a member of the family Sapindaceae and is an exotic species for Turkey. It is a kind of tree that can grow up to an average of 15-20 m and is deciduous, which we often see in parks, gardens and roadsides. Although it is not in the natural flora of Turkey due to the use of different areas especially in the city centers, it is being used in the areas where many different climate types dominate. In this study, it was aimed to determine the variation of some micromorphological characters of the leaves of Aesculus hippocastanum L. depending on the growing environment. Samples were grown on 6 different cities which are dominated by different climate types. With this purpose, leaf samples were collected from Aesculus hippocastanum L. individuals growing in 6 different cities (Rize, Samsun, İzmir, Antalya, Sivas and Ankara) in the Central and Western Anatolia regions where the Black Sea, Mediterranean and continental climate types predominate. Scale images were obtained with the help of electron microscope (SEM = Scanning Electron Microscope) of the collected leaf samples and stoma length (μm), stoma width (μm), por length (μm), por width and stoma density (per mm field) were determined. Variance analysis and Duncan test were applied to the obtained data with the help of SPSS package program and the variation of the indicated characters according to the growing environment was evaluated statistically. As a result of the study, it was determined that there was a statistically significant difference in at least 95% confidence level among the growth environment conditions for all characters except for stoma width.

Variation of some micro-morphological characters of leaves of Aesculus hippocastanum based on growing environment