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Rotaviruses belong to the genus Rotavirus within the Reoviridae family and its genome consists of eleven dsRNA segments. These viruses are distinct in that their segmented genome undergoes reassortment during replication.1 According to the classification system based on the gene sequence of VP6 an inner capsid protein, rotaviruses are currently categorized into nine groups (A-I).2 Rotavirus group A is a major etiologic agent of acute gastroenteritis in children under 5 years of age and is associated annually with approximately 215,000 deaths worldwide.3 Rotaviruses group A are classified based on the molecular characterization of the two outer capsid proteins, VP7 (glycoprotein) and VP4 (protease-sensitive) into different G and P genotypes respectively.4 At least 36 G genotypes and 51 P genotypes of group A rotavirus had been reported to cause . ABSTRACT

international journal of current research and review

Monoplex Nested and Semi-nested Reverse Transcription Polymerase Chain Reaction for G and P Genotyping of Human Rotavirus Group A in Clinical Specimens and Environmental Samples