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Hematologic neoplasm is listed in the top ten malignancies worldwide and also one of the leading causes of mortality in patients with cancer. Almost 9% of all cancer cases diagnosed in a year are hematological malignancies.2,3 Identification of clonal aberrations yields support in diagnosing malignant/premalignant diseases as well as gives important information regarding prognosis and therapy.4,5 Therefore, conventional cytogenetic analysis is mandatory in the evaluation of suspected acute leukemia. It is one of the essential tools for classification of hematological malignancies, prognostication and treatment.6,7 It is also seen that unsuccessful conventional karyotyping has a prognostic implication in hematological malignancies.8,9,10

Challenges of Pre-analytical Variables in Conventional Cytogenetics - A University Teaching Hospital Experience