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This study investigated the effect of various concentrations of pteroyl-mono-γ-glutamate (PteGlu 1 ) and pteroyl-di-γ-glutamate (PteGlu 2 ) on the growth of Lactobacillus rhamnosus strains ATCC 7469 (wild-type strain) and ATCC 27773 (chloramphenicol-resistant strain) used for folate microbiological assays. At concentrations of 0.025-0.20 nmol/L, the growth of the chloramphenicol-resistant strain was stimulated to a greater extent by PteGlu 1 than by PteGlu 2 , but the wild-type strain did not show such phenomena. L. rhamnosus ATCC 27773 bioassays were used to determine the total folate content of various foods treated with a chicken pancreas folate conjugase. This showed a significantly lower value when PteGlu 1 was used as a calibrator than with PteGlu 2 . These results indicated that PteGlu 2 should be the standard folate compound when chicken pancreas folate conjugase is used in preparing samples for L. rhamnosus ATCC 27773 bioassay.

Growth Response of <i>Lactobacillus rhamnosus</i> Chloramphenicol-Resistant Strain ATCC 27773 to Pteroyl-Mono- and -Di-Glutamates