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For determination of selenium (Se) in biological materials, an improved method based on high performance liquid chromatographic determination of the fluorophore formed by reaction of selenite with 2,3-diaminonapththalene was developed. The concentration detection limits were 0.5 ng/g in dried materials and 0.03 ng/mL in fluid materials. In quadruplicate assays of 11 biological reference materials using the proposed method, measured Se concentrations were not significantly different from their certified values. Thus, the proposed method is reliable and suitable for the determination of trace levels of Se in foods. Using the proposed method, Se concentrations in various kinds of tea were determined to assess the contribution of tea to daily Se intake in the Japanese population. Se concentration in the leaves of general black, green and oolong tea obtained in local retail stores was 33 +/- 19 ng/g (n=440). The leaves of a particular Chinese green tea sold under the name "high Se tea" were found to contain 455 +/- 184 ng/g (n= 14) of Se. While the percentage of Se extractable by infusion was less than 5% for the general teas, that in the high Se tea was more than 20%. These results indicated that intake of tea does not contribute to daily Se intake in the Japanese population. However, since infusions from high Se tea contained over 5 ng/mL of Se, consumption of over 1 L/d of tea derived from such high Se teas may increase the daily Se intake by close to 10%.

Quantitative Evaluation of Selenium Contained in Tea by High Performance Liquid Chromatography.