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Background:   The present study aimed to evaluate the in vitro and in situ antagonistic effects of Lactobacillus probiotic strains on clinical strains of  Helicobacter pylori . Also to investigate their immunomodulation effects on a macrophage cell model.Materials and Methods:   Anti-microbial effects of probiotic lactobacilli against  H. pylori  was assessed using the well and disk diffusion methods. Effects of lactobacilli probiotics strains, as well as their cell-free supernatant on adhesion of  H. pylori  to MKN-45 gastric epithelial cells, were examined in their presence and absence. Immunomodulation effects of probiotic lactobacilli were performed using the U937 macrophage cell model. Incubation of host cells with probiotics and their cell-free supernatants with cultured host cells was performed in different optimized conditions. The supernatant of host cells cultured in their presence and absence was used for cytokines measurement.Results:   Two probiotics, Lactobacillus acidophilus  ATCC4356, and  Lactobacillus rhamnosus  PTCC1607, could inhibit the growth of clinical  H. pylori  in vitro. They could also inhibit attachment of  H. pylori  to MKN-45 cells. Cell-free supernatant of  L. acidophilus  had a stimulating effect on the production of Interferon-gamma (IFN-γ) by U937 cells.Conclusion:   The present study demonstrates that,  L. acidophilus  ATCC4356 and  L. rhamnosus  PTCC1607 probiotic strains can inhibit the growth of clinical  H. pylori  in vitro. Treatment of U937 with alive  H. pylori  plus cell-free supernatant of  L. acidophilus , have a significantly higher capacity to stimulate IFN-γ production than  H. pylori  alone. So, the metabolite (s) of this probiotic may have an immunomodulatory effect in immune response versus  H. pylori .

Antagonistic and Immunomodulant Effects of Two Probiotic Strains of Lactobacillus on Clinical Strains of Helicobacter pylori