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As a result of city, industrial and agricultural activities, a large amount of wastewater is generated every year. The wastewater generated must be treated. When the nitrogen concentration in wastewater reaches more than 1.9 mg / L, favorable environmental conditions are provided for the microalgae to multiply. In the presence of microalgae in wastewater treatment technologies, treatment has gained importance in recent years and has become a more environmentally-friendly alternative in treatment. Research on the availability of biomass as a source of raw materials in the production of alternative energy sources is increasingly important. In this paper, Chlorella variabilis microalgae growth, lipid productivity and nutrient removal in wastewater media were investigated. Before inoculation of microalgae, wastewater was centrifuged at 4000 rpm to remove the solid particles and was diluted with tap water in different ratios (0-40%). Wastewaters were inoculated with Chlorella variabilis in 250 mL open flasks in a 200 rpm shaking incubator for a month at 27 °C. After incubation maximum cell concentration (Xmax=1.03 gdw/L), growth rate (µmax=4.0 x10-3), doubling time (173 h) of the microalgae were reached in 40% diluted medium. Fat content (21%) and lipid productivity (6x10-3 g/L.d) were determined concurrently for 20% diluted media. It was determined that microalgae lipids were rich in oleic (C18:1 38%) and linolenic acid (C18:3 35%). The efficiency of COD (Chemical oxygen demand) and total phosphor removal in the presence of microalgae had been almost 60% and 77% respectively. According to the experimental results, the treatment of wastewater in the presence of microalgae is promising for future applications.

Treatment of fizzy drink factory wastewaters by microalgae and evaluation of algae oil for biofuel production