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In this study, we prepared a novel fluorescent chemosensor containing an imidazole molecule and the chemosensor characterized utilizing 1 H-NMR, 13 C-NMR spectroscopy, FT-IR spectrometer and elemental analyzer. Prepared sensor was utilized as an effectively selective and a fastly responsive chemical fluorescent sensor for ‘’turn off’’ determination of mercury (II) ions in EtOH. A clear complex between 2-((4-(1H-phenanthro [9,10-d] imidazol-2-yl)benzylidene)amino) phenol (PENIM) and Hg + ions was determined and calculated employing the Job’s method and also the limit of detection value was found to be 2.1 nM on the basis of 3 σ / k . Furthermore, the sensor-Hg 2+ displayed and reversible property for mercapto containing cysteine molecules. Also, the fluorescence enhancement and quenching studies were supported by computational experiments based on the density functional theory (DFT) calculations.

A Turn OFF Fluorescent Probe For Selective Detection Of Hg2+ Ions