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In order to investigate vascular diseases such as cause of atherosclerosis and myocardial infarction, it is important to investigate relationships between endothelial cells (ECs) covered with surface on blood vessels and blood flow stimulation using both of in vivo and in vitro experiments. In this paper, a blood vessel model for in vitro experiment was developed in order to evaluate the relationship between response of ECs and shear stress caused by blood flow. ECs were cultured in polydimethylsiloxane (PDMS) microchip, which made of (PDMS) microchip fabricated using usual soft-lithographic technique, by optimizing cells cultured condition such as surface coating on the wall. The microchip consists of a straight microchannel with 400 μm width, 100 μm depth and 2 cm long. It was confirmed to be confluent using a phase-contrast microscope. Velocity distributions to estimate shear stress on the ECs were measured using micro PIV system.

Velocity measurement of a blood vessel model cultured endothelial cells using micro PIV technique