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A simple colorimetric biosensor, which uses the modified Ellman’s reaction, enables selective analysis of nerve agents based on a different ability of bispyridinium oximes to reactivate enzyme-inhibitor complexes in the phase before dealkylation. The analysis was made based on spectral data of reflectance of the surface of a cotton cloth reaction zone of biosensor with immobilized and stabilized enzyme after inhibition and subsequent reactivation. The evaluation of measured data was made based on a method of artificial neuronal networks. The individual inhibitors from the groups of three nerve agents were identified: sarin, soman, tabun, cyclosarin, agent VX and its Russian analogue, R-33.

ANALYSIS OF NERVE AGENTS THROUGH A MODIFIED METHOD OF CHOLINESTERASE ASSESSMENT USING ARTIFICIAL NEURONAL NETWORKS