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Age-dependent humoral immune response of barfin flounder Verasper moseri was investigated. One hundred μL of recombinant coat protein of barfin flounder nervous necrosis virus (BFNNV) adjusted to 250 μg/mL in Tris-EDTA buffer (pH 8.0) was injected intravascularly to 4, 5, 6 and 7 month-old fish. One month after injection, an enzyme-linked immunosorbent assay (ELISA) antibody titer was measured. Although no ELISA antibody titers were detected in injected or noninjected fish of 4 and 5 month-old, the antibody titers were remarkably increased (p<0.025) in 6 and 7 month-old fish. High ELISA antibody titers were kept for at least 2 months.  マツカワの液性免疫応答開始時期の検討を行った。 47ケ月齢魚の尾部血管内に250μg/mlのタンパク質量に調整した大腸菌発現 BFNNV 外被タンパク質32kDa を　100μL注射した。 抗原投与後1ヶ月毎に ELISA を用いて血清中の抗体価を測定した。 5ヶ月齢以下の魚では投与の前後で ELISA 抗体価に違いは認められなかったが 6ヶ月齢以降の魚では明瞭な ELISA 抗体価の上昇が観察された。 高いELISA 抗体価は抗原投与2ヶ月間にわたって維持された

Age-dependent Humoral Immune Response of Barfin Flounder against Recombinant Coat Protein of Barfin Flounder Nervous Necrosis Virus.