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Enzyme linked immunosorbent assay (ELISA) was applied to detect the antibody of Japanese flounder (Paralichthys olivaceus) against lymphocystis disease virus (LCDV). Purified Japanese flounder LCDV was used as a capture antigen of ELISA. Monitoring the immune response of Japanese flounder to LCDV was done using the sera obtained from apparently healthy, LCD-diseased and LCD-recovered fish. ELISA absorbances of these three groups were clearly different. Using the serum with high ELISA absorbance, the optimum ELISA condition was set up. Apparently healthy fish were injected with inactivated LCDV. One to three months after the injection, the antibody against LCDV was detected by ELISA established for LCDV and their ELISA antibody titer increased during that period. 　精製リンホシスチス病LCDウイルスを ELISA 抗原として, ヒラメ健康魚, 発病魚および感染耐過魚の血中抗体検出を試みたところ, 群れごとに ELISA 吸光値に差が認められ, 値は上記の順に高かった。 感染耐過魚の血清を用い, 抗原および各抗血清の最適濃度ならびに ELISA のベースラインを決定した。 健康魚に不活化ウイルスを接種し, 最適 ELISA 条件下で血中抗体価を測定したところ, 接種13か月後に抗体価の上昇が観察され, 本 ELISA は免疫後の血中抗体価を測定するのに有効であると考えられた

Detection of Antibody against Lymphocystis Disease Virus in Japanese Flounder by Enzyme Linked Immunosorbent Assay.