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Six peanut genotypes grown at three locations (sources) were tested in laboratory studies for the influence of pod and seed inoculation methods on seed colonization by Aspergillus parasiticus Speare and/or incidental prior contamination (uninoculated method) in the field or storage by A. flavus Link ex Fr. or A. parasiticus. The genotypes has been identified as having varying levels of resistance to seed colonization by Aspergillus spp. in laboratory screening. There was no difference between the pod and seed inoculation on the mean percentage of seed colonization, but pod inoculation resulted in a noticeable reduction in seed colonization of the more susceptible genotypes when compared to inoculated seed (genotype x method interaction). Uninoculated seed incubated similarly to the inoculated samples exhibited considerably less colonization. For all three methods, seed colonization was consistently less for the resistant genotypes than for the ‘Florunner’ variety or the highly susceptible check P. I. 343419. A source × genotype interaction resulted from the difference in the magnitude of percent colonization but the resistant genotypes were colonized less frequently than susceptible genotypes. The seed screening method currently in use (seed inoculation) was equally or more effective than the pod inoculation or uninoculated seed method in identifying genotypes resistant to A. parasiticus.

Comparison of Pod and Seed Screening Methods on Aspergillus spp. Infection of Peanut Genotypes1