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This study evaluated the bactericidal activity of the cationic surface active compound didecyldimethylammonium chloride in 2-propanol against the two reference strains  Pseudomonas aeruginosa  and  Pseudomonas aeruginosa  (tetracycline-resistant) as well as their isolates adapted to this active substance. Individual phases and stages of European Standards which are intended to determine the bactericidal activity of disinfectants in medical areas were used. Disinfection parameters of the tested substance as determined by suspension methods were not sufficient to achieve the required bactericidal reduction in the carrier method for  Pseudomonas aeruginosa  and all its adapted isolates.  Pseudomonas aeruginosa  (tetracycline-resistant) and its adapted isolates were more sensitive to the tested active substance when the contact time was extended from 5 to 10/15 min. Adaptive resistance of  Pseudomonas aeruginosa  strains can be abolished by using increased concentrations of the tested substance and/or extended contact time.

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Evaluation of the Bactericidal Activity of Didecyl Dimethyl Ammonium Chloride in 2-Propanol against  Pseudomonas aeruginosa  Strains with Adaptive Resistance to this Active Substance According to European Standards