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Thin – layer chromatography of fifteen surfactants (cationic, anionic and non – ionic) has been performed on silica HPTLC plates using chromogenic reagents methylene blue (MB), bromocresol green (BCG), and bromophenol blue (BPB) in the presence of aqueous formic acid plus acetone. Mobile phase system comprising acetone + 10 −4  M bromophenol blue in 1 M aqueous formic acid is found most favourable for the separation of coexisting, polyoxyethylene p-t-octylphenol (TX-100), polyoxyethylene(20) stearyl ether (Bj-78), and dodecyltrimethylammonium bromide (DTABr). Effects of sample pH, presence of impurities in the analyte sample, nature of adsorbent and the composition of mobile phase have been investigated on the separation of TX-100, Bj-78, and DTABr from their mixtures. The limits of detection of poly oxyethylene p-t-octylphenol, polyoxyethylene(20) stearyl ether and dodecyl trimethyl ammonium bromide have been determined.

tenside surfactants detergents

On Plate Analysis of Surfactants by High Performance Thin-Layer Chromatography: Simultaneous Identification and Separation of Polyoxyethylene p-t-Octyl Phenol, Dodecyltrimethylammonium Bromide and Polyoxyethylene(20) Stearyl Ether