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The methanol extract of the aerial part of Daucus carota var. sativus showed a stronger antioxidative activity than the standard synthetic antioxidant, 3-tert-butyl-4-hydroxyanisole (BHA). From this extract, five known compounds, luteolin 7-O-glucopyranoside (1), chrysoeriol 7-O-glucopyranoside (2), chlorogenic acid (3), maltol 3-O-glucopyranoside (4) and benzyl -glucopyranoside (5) were isolated. Among them, when 1–4 were investigated for their antioxidative activity using the ferric thiocyanate method, 1–3 indicated an antioxidative activity. The scavenging effect of 1–4 on the stable free radical, 1,1-diphenyl-2-picrylhydrazyl was also examined. Compounds 1 and 3 showed a scavenging effect. In addition, 1–3 were assayed for their inhibitory effects on the activation of inactive hyaluronidase induced by compound 48/80. All tested compounds showed this effect.

Antioxidative and Antihyaluronidase Activities of Some Contituents from the Aerial Part of Daucus carota.