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Antimicrobial substances were isolated from the 50% ethanol extract of paprika seeds by ODS open column chromatography and reverse phase HPLC. Ten compounds (1-10) which demonstrated antimicrobial activity against Saccharomyces cerevisiae (MIC=1.3-2.6 mg/l) were isolated. The structure of the major compound, 4 (MIC=2.6 mg/l), was determined to be 3β-O-{β-D-glucopyranosyl-(l→3)-β-D-glucopyransyl-(1→3)-[β-D-glucopyranosyl-(1→2)]-β-D-glucopyransyl-(1→4)-β-D-galactopyranosy}-2α-hydroxyl-(25R)-5α-spirostane by MS, NMR and sugar analyses.

Isolation and Structure of Antimicrobial Substances from Paprika Seeds.