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Lipids extracted from two cultivars of Jack bean (Canavalia gladiata DC.) were classified by thin-layer chromatography (TLC) into nine subfractions. The major lipid components were triacylglycerols (TAG: 43.8 − 45.7%) and phospholipids (PL: 46.7 − 47.0%). Other lipids were present at a concentration of 0.3 − 2.7%. The molecular species and FA distributions of TAG, isolated from the total lipids in the beans, were analyzed by a combination of AgNO3-TLC and gas chromatography (GC). Eighteen TAG molecular species were detected in the beans. The dominant TAG species were M3 (32.5 − 33.0%), followed by S2M (5.4 − 5.5%), SM2 (7.6 − 7.7%), S2D (9.0 − 9.1%), SMD (5.3 − 5.4%), S2T (6.3 − 6.4%), M2D (5.3 − 5.6%), MD2 (6.5 − 6.7%), SDT (5.2 − 5.3%) and M2T (3.8 − 3.9%), where S, M, D, and T denote saturated FA, monoene, diene, and triene, respectively. The results show that the lipid classes, FA distributions and TAG molecular species of Jack bean are not dependent on the cultivar.

Characteristics of Lipid Components and Triacylglycerol Molecular Species of Jack Bean (Canavalia gladiata DC.)