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Defatted rice bran was treated at 230°C for 5 min with distilled water or 40% (v/v) acetone to produce the corresponding extracts. Acetone-soluble substances were fractionated from the extracts. The antioxidative activities against 1,1-diphenyl-2-picrylhydrazyl, hydroxyl and peroxyl radicals, and the hypochlorite and peroxynitrite ions of the extracts and acetone-soluble substances were examined, and the activities were comprehensively characterized using the 5-axe cobweb chart. The 40% acetone extract exhibited higher activity than the water extract. Fractionation of acetone-soluble substances from extracts increased activity. The acetone-soluble substances from the extract with 40% acetone exhibited the highest activity among the extracts and acetone-soluble substances.

Evaluation of Antioxidative Activity for Extracts from Defatted Rice Bran Using 5-Axe Cobweb Chart